Hepatoprotective Effects Of Papaya Leaves Extract (Carica Papaya L.) With Increased Dose: A Study Of Total Bilirubin Levels Of Wistar Rats That Given Paracetamol

Authors

  • Adhani Rihhadatul 'aisy Faculty of Medicine, Muhammadiyah University of Semarang, Semarang, Indonesia
  • Kanti Ratnaningrum Laboratorium Biomedik, Faculty of Medicine, Muhammadiyah University of Semarang, Semarang, Indonesia
  • Dyah Mustika Nugraheni Laboratorium Biomedik, Faculty of Medicine, Muhammadiyah University of Semarang, Semarang, Indonesia

DOI:

https://doi.org/10.12928/admj.v2i2.5063

Keywords:

Papaya leaves; Ethanolic Extract; Total bilirubin levels; Paracetamol; Hepatoprotective

Abstract

Incorrect use of paracetamol can cause overdose and cause liver damage. Papaya leaves is a plant that has the potential as hepatoprotective because it contains flavonoids, saponins, tannins, alkaloids, triterpenoids and vitamin E. The aim of this study was to prove the hepatoprotective effect of papaya leaf extract on the total bilirubin levels of Wistar rats given paracetamol. This research was conducted using a post test only control group design method and used 24 wistar rats grouped by simple random sampling into negative control (K-), positive control (K +), treatment 1 (PI), and treatment 2 ( PII). PI and PII groups were given papaya leaf extract 75 mg / kgBB rats / day and 150 mg / kgBB rats / day for 7 days. On days 5 to 7, the K +, PI, and PII groups were given paracetamol 600 mg / kgBW rats / day. Samples were taken on day 8 and total bilirubin levels were measured on day 9 then analyzed using One Way Anova and Post Hoc LSD test. There are significant differences in all groups based on the results of the One Way Anova test with p value= 0.000. The results of the LSD Post Hoc test showed significant differences in the K- group with K + (p = 0,000), K- with PI (p = 0.027), K + with PI (p = 0,000), K + with PII (p = 0,000), PI with PII (p = 0.004), and there was no significant difference between the K- and PII groups (p = 0.397). There was a hepatoprotective effect of papaya leaf extract on Bilirubin total levels in Wistar rats treated with paracetamol.

References

Afandi, M. (2017). Uji Antiinflamasi Ekstrak Etanol Daun Pepaya (Carica papaya Linn) Pada Tikus

Putih Jantan Galur Wistar yang Diinduksi Karagenin. Surakarta.

Boorman G, Suttie A, Leininger J, Eustis S, Elwell M, Bradley A, MacKenzie W. (2017). Boorman's

Pathology of the Rat. 2nd edition. Academic Press.

Hardiningtyas, S. D., Purwaningsih, S., & Handharyani, E. (2014). Aktivitas antioksidan dan Efek

Hepatoprotektif Daun Bakau Api-Api Putih. JPHPI, 17(1), 80-91.

https://doi.org/10.17844/jphpi.v17i1.8140

Hasan IL. (2014). Drug-Induced Liver Injury: Tantangan dalam Diagnosis. Contin. Med. Educ,

(3), 167-170.

Kamilah BR, Aminullah, A., Hadisaputro, S., Soemantri, A., & Suhartono, S. (2012). Kadar Oksidan

yang Tinggi Sebagai Faktor Risiko Terjadinya Hemolisis Pada Neonatus Sepsis. Sari Pediatri,

(3), 198–204. https://doi.org/10.14238/sp14.3.2012.198-204

Kasminah. (2016). Aktivitas Antioksidan Rumput Laut Halymenia durvillae dengan Pelarut Non

Polar, Semi polar dan Polar (Skripsi, Universitas Airlangga, Surabaya).

Lwanga S & Lameshow S. (2011). Sample Size Determintation For Health Study: A Practical

Manual, Geneva: WHO.

Mahatriny, N. N., Payani, N. P. S. , Oka, I. B. M. , Astuti, K. W. (2014). Skrining Fitokimia Ekstrak

Etanol Daun Pepaya (Carica papaya L.) yang Diperoleh dari Daerah Ubud, Kabupaten

Gianyar, Bali. Jurnal Farmasi Udayana, 3(1), 8-13

Qurrota, A., & Laily, A. N. (2015). Analisis Fitokimia Daun Pepaya (Carica papaya L.) di Balai

Penelitian Tanaman Aneka Kacang dan Umbi , Kendalpayak , Malang. 134-137.

Rehatta, N., Hanindito, E., Tantri, A., Redjeki, I., Soenarto, R., Bisri, D., Lestari, M. (2019).

Anestesiologi dan Terapi Intensif Buku Teks KATI-PERDATIN. Jakarta: PT Gramedia Pustaka

Utama.

Riset Kesehatan Dasar. (2010). Jakarta: Departemen Kesehatan Republik Indonesia.

Rosida, A. (2016). Pemeriksaan Laboratorium Penyakit Hati. Berkala Kedokteran, 12(1), 123–131.

Saputri, D. (2013). Pengaruh Pemberian Ekstrak Aseton Daun Pepaya (Carica papaya, L.) Sebagai

Hepatoprotektor Pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol (Skripsi,

Universitas Sebelas Maret, Surakarta).

Silvani, F. N., Sukohar, A., & Rudiyanto, W. (2019). Pengaruh Ekstrak Etanol Belimbing Wuluh

(Averrhoa bilimbi Linn) Sebagai Antioksidan Terhadap Histopatologi Hepar Tikus Galur

Sprague dawley yang Diinduksi Parasetamol. Majority, 8(1), 95-101.

Sunarni, T., Prastiwi, R., & Rinanto, Y. (2013). Kombinasi Ekstrak Etanol Buah Mengkudu (Morinda

citrifolia L.) dan Daun Pepaya (Carica papaya L.) Sebagai Hepatoprotektif Selama Pengobatan

Tuberkulosis. Ilmu Kefarmasian Indonesia, 11(2), 160-166

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Published

2021-12-30

How to Cite

’aisy, A. R., Ratnaningrum, K., & Nugraheni, D. M. (2021). Hepatoprotective Effects Of Papaya Leaves Extract (Carica Papaya L.) With Increased Dose: A Study Of Total Bilirubin Levels Of Wistar Rats That Given Paracetamol. Ahmad Dahlan Medical Journal, 2(2), 46–57. https://doi.org/10.12928/admj.v2i2.5063