Phytoremediation Potential of Azolla microphylla and Salvinia molesta for Copper (Cu) Removal: Effects on Biomass, Antioxidant Activity, and DNA Integrity
DOI:
https://doi.org/10.12928/jbns.v6i1.16334Keywords:
Azolla microphylla, Copper (Cu), DNA damage, Salvinia molesta, Superoxide dismutase (SOD)Abstract
Industrial wastewater represents a major source of environmental pollution, primarily due to the presence of heavy metals that pose significant risks to human health. Copper (Cu) is an essential trace element; however, its occurrence at elevated concentrations in aquatic environments can exert toxic effects. The aquatic plants Azolla microphylla and Salvinia molesta are known to possess potential as phytoremediation agents for heavy metal contamination. This study aimed to evaluate the effectiveness of these plants in reducing Cu concentrations, to quantify biomass production, to analyze the activity of the enzyme superoxide dismutase (SOD), to detect DNA damage using the comet assay, and to assess changes in water quality following treatment. Phytoremediation was conducted using a static system at Cu concentrations of 0, 10, 25, 50, 75, and 100 ppm. Data were analyzed using one-way ANOVA at a 95% confidence level. The results demonstrated that Azolla microphylla reduced Cu concentrations by up to 95.32%, whereas Salvinia molesta achieved a reduction of 72.42%. Plant dry biomass tended to decrease with increasing Cu concentrations, while metal accumulation within plant tissues increased. SOD enzyme activity did not show a significant increase, and no DNA damage was detected in leaf tissues, indicating the absence of severe oxidative stress. Furthermore, dissolved oxygen (DO) levels increased after 14 days of treatment. In conclusion, Azolla microphylla and Salvinia molesta exhibit strong potential as effective phytoremediators for Cu heavy metal contamination in aquatic environments.
References
Ali, H., Khan, E., & Sajad, M. A. (2013). Phytoremediation of heavy metals: Concepts and applications. Chemosphere, 91(7), 869–881. https://doi.org/10.1016/j.chemosphere.2013.01.075
Alkorta, I., Hernández-Allica, J., Becerril, J. M., Amezaga, I., Albizu, I., & Garbisu, C. (2004). Recent findings on the phytoremediation of soils contaminated with environmentally toxic heavy metals and metalloids. Reviews in Environmental Science and Biotechnology, 3, 71–90. https://doi.org/10.1023/B:RESB.0000040059.70899.3d
Alscher, R. G., Ertürk, N., & Heath, L. S. (2002). Role of superoxide dismutase in controlling oxidative stress in plants. Journal of Experimental Botany, 53(372), 1331–1341. https://doi.org/10.1093/jxb/53.372.1331
Apel, K., & Hirt, H. (2004). Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
Araoye, P. A. (2009). The seasonal variation of pH and dissolved oxygen (DO₂) concentration in Asa Lake Ilorin, Nigeria. International Journal of Physical Sciences, 4(5), 271–274.
Asih, D. W., & Rachmadiarti, F. (2019). Azolla microphylla sebagai fitoremediator logam Pb. LenteraBio, 8(1), 85–90.
Astuti, S. N. C. T., & Nugroho, A. P. (2022). Toxicity of copper (Cu) and chromium (Cr) on the seed germination of mung bean (Vigna radiata L.). Journal of Biotechnology and Natural Science, 2(2), 40–48. https://doi.org/10.12928/jbns.v2i2.6961
Astuti, S. N. C. T., Aisah, S., & Solihah, J. (2023). The potential of Salvinia molesta as a copper phytoremediation agent based on gene expression analysis. Journal of Biotechnology and Natural Science, 3(1), 30–40. https://doi.org/10.12928/jbns.v3i1.9739
Chaney, R. L., Malik, M., Li, Y. M., Brown, S. L., Brewer, E. P., Angle, J. S., & Baker, A. J. M. (1997). Phytoremediation of soil metals. Current Opinion in Biotechnology, 8(3), 279–284. https://doi.org/10.1016/S0958-1669(97)80004-3
Chang, W. Y. B., & Ouyang, H. (1988). Dynamics of dissolved oxygen and vertical circulation in fish ponds. Elsevier.
Chen, J., Liu, Y., Yan, X., Wei, G., Zhang, J., & Fang, L. (2018). Rhizobium inoculation enhances copper tolerance in Medicago sativa. Ecotoxicology and Environmental Safety, 162, 312–323. https://doi.org/10.1016/j.ecoenv.2018.07.001
Clemens, S. (2006). Toxic metal accumulation, responses to exposure and mechanisms of tolerance in plants. Biochimie, 88(11), 1707–1719. https://doi.org/10.1016/j.biochi.2006.07.003
Cobbett, C. S. (2000). Phytochelatins and their roles in heavy metal detoxification. Plant Physiology, 123(3), 825–832. https://doi.org/10.1104/pp.123.3.825
Collins, A. R. (2014). Measuring oxidative damage to DNA and its repair with the comet assay. Biochimica et Biophysica Acta, 1840(2), 794–800. https://doi.org/10.1016/j.bbagen.2013.04.022
DalCorso, G., Farinati, S., & Furini, A. (2008). Regulatory networks of cadmium stress in plants. Plant Signaling & Behavior, 3(8), 663–667. https://doi.org/10.4161/psb.3.8.5906
Darmono. (1995). Logam dalam biologi makhluk hidup. Universitas Indonesia Press.
Fitter, A. H., & Hay, R. K. (2001). Fisiologi lingkungan tanaman. UGM Press.
Gill, S. S., & Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance. Plant Physiology and Biochemistry, 48(12), 909–930. https://doi.org/10.1016/j.plaphy.2010.08.016
Hasanuzzaman, M., Hossain, M. A., da Silva, J. A. T., & Fujita, M. (2012). Plant response and tolerance to oxidative stress. In V. Bandi et al. (Eds.), Crop stress and its management (pp. 261–315). Springer.
Jafari, N., Senobari, Z., & Pathak, R. K. (2010). Biotechnological potential of Azolla spp. for biosorption of heavy metals. Ecology and Environmental Conservation, 16, 443–449.
Knopper, L. D., & McNamee, J. P. (2008). Use of the comet assay in environmental toxicology. In Methods in Molecular Biology (Vol. 410, pp. 171–183). https://doi.org/10.1007/978-1-59745-548-0_11
Lakitan, B. (2015). Dasar-dasar fisiologi tumbuhan. RajaGrafindo Persada.
Mangkoediharjo, S., & Samudro, G. (2010). Fitoteknologi terapan. Graha Ilmu.
Marklund, S., & Marklund, G. (1974). Involvement of superoxide anion radical in autoxidation of pyrogallol. European Journal of Biochemistry, 47(3), 469–474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7(9), 405–410. https://doi.org/10.1016/S1360-1385(02)02312-9
Naghipour, D., Ashrafi, S. D., Gholamzadeh, M., Taghavi, K., & Naimi-Joubani, M. (2018). Phytoremediation of heavy metals by Azolla filiculoides. Data in Brief, 21, 1409–1414. https://doi.org/10.1016/j.dib.2018.10.115
Palar, H., & Haryando. (2012). Pencemaran dan toksikologi logam berat (Edisi revisi). Rineka Cipta.
Parmar, P., Dave, B., Sudhir, A., Panchal, K., & Subramanian, R. B. (2013). Plant response against heavy metal stress. International Journal of Current Research, 5(1), 80–89.
Prasad, M. N. V., & Freitas, H. (2003). Metal hyperaccumulation in plants. Electronic Journal of Biotechnology, 6(3), 285–321. https://doi.org/10.2225/vol6-issue3-fulltext-6
Rahman, M. A., & Hasegawa, H. (2011). Aquatic arsenic phytoremediation. Chemosphere, 83(5), 633–646. https://doi.org/10.1016/j.chemosphere.2011.02.045
Rai, P. K. (2008). Phytoremediation using Azolla pinnata. International Journal of Phytoremediation, 10(5), 430–439. https://doi.org/10.1080/15226510802100606
Rosmarkam, A., & Yuwono, N. W. (2002). Ilmu kesuburan tanah. Kanisius.
Sharma, P., Jha, A. B., Dubey, R. S., & Pessarakli, M. (2012). ROS and antioxidative defense in plants. Journal of Botany, 2012, 1–26. https://doi.org/10.1155/2012/217037
Srivastava, S., Mishra, S., Tripathi, R. D., Dwivedi, S., & Gupta, D. K. (2006). Copper-induced oxidative stress in Hydrilla verticillata. Aquatic Toxicology, 80(4), 405–415. https://doi.org/10.1016/j.aquatox.2006.09.007
Talebi, M., Tabatabaei, B. E. S., & Akbarzadeh, H. (2019). Hyperaccumulation of metals in Azolla species. Chemosphere, 230, 488–497. https://doi.org/10.1016/j.chemosphere.2019.05.098
Wang, Y., Pennock, S. D., Chen, X., Kazlauskas, A., & Wang, Z. (2004). Signal transduction mechanisms. Journal of Biological Chemistry, 279(9), 8038–8046. https://doi.org/10.1074/jbc.M311405200
Yadav, S. K. (2010). Heavy metal toxicity in plants. South African Journal of Botany, 76(2), 167–179. https://doi.org/10.1016/j.sajb.2009.10.007
Yruela, I. (2005). Copper in plants: Acquisition, transport and interactions. Functional Plant Biology, 32(5), 409–430. https://doi.org/10.1071/FP05016
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