Effect of Combined Kelakai (Stenochlaena palustris) Leaves Extract and Stingless Bee Honey on Hemoglobin Levels in Mice (Mus musculus)
DOI:
https://doi.org/10.12928/jbns.v6i1.15802Keywords:
Anemia, Hemoglobin level, Mus musculus, Stenochlaena palustris, Stingless bee honeyAbstract
Anemia is a hematological condition characterized by decreased hemoglobin (Hb) levels, leading to impaired oxygen transport and reduced physiological function. Natural products such as kelakai leaves (Stenochlaena palustris) extract and stingless bee honey have potential as complementary therapies for anemia due to their iron content and bioactive compounds, including flavonoids and antioxidants that may support erythropoiesis. This study aimed to evaluate the effect of a combination of kelakai leaves extract and stingless bee honey on hemoglobin levels in mice (Mus musculus). A true experimental study with a pretest–posttest control group design was conducted using 30 male mice divided into six groups: normal control, untreated anemic control, anemic group treated with iron supplementation, and three treatment groups receiving combinations of kelakai leaves extract and stingless bee honey at ratios of 30:70, 50:50, and 70:30. Anemia was induced using sodium nitrite, and hemoglobin levels were measured before and after treatment. Data were analyzed using one-way analysis of variance (ANOVA) followed by post hoc tests. The results demonstrated that sodium nitrite induction successfully reduced hemoglobin levels below normal, while all treatment groups showed a significant increase in hemoglobin levels compared to the untreated anemic group (p < 0.05). The highest mean hemoglobin level was observed in the 70:30 combination group (16.36 g/dL), whereas the lowest was found in the untreated anemic group (10.22 g/dL). In conclusion, the combination of kelakai leaves extract and stingless bee honey effectively increased hemoglobin levels in mice, with the 70:30 ratio identified as the most effective formulation.
References
Agustina D. (2017). Pemanfaatan Daun Kelakai Sebagai Teh Penambah Darah. Jurnal Ilmiah Kanderang Tingang, 8(1), 8–10.
Astuti, E. R. (2023). Literature Review: Faktor-Faktor Penyebab Anemia Pada Remaja Putri. Jambura Journal of Health Sciences and Research, 5(2), 550–561. https://doi.org/10.35971/jjhsr.v5i2.17341
Azkiyah, S. Z., Rahmaniyah, D. N. K., Istiana, & Wafiyah, I. (2021). The effect of Giving Vitamin C on the Iron (Fe) Absorption of Anemic Mice (Mus musculus) by Sodium Nitrite Induction. Jurnal Farmasi Tinctura, 2(2), 79–86.
Chaparro, M., C., Suchdev, & S., P. (2019). Anemia epidemiology, pathophysiology, and etiology in low‐ and middle‐income countries. Annals of the New York Academy of Sciences, 1450(1), 15–31. https://doi.org/10.1111/nyas.14092
Dahliansyah, & Petrika Yanuarti. (2020). Pemberian Madu Heterotrigona itama. (Stingless bee) Dan Sari Jeruk Siam Sambas Terhadap Kadar Hemoglobin Darah (Hb) Ibu Hamil. Jurnal Surya Medika, 6(1), 157–162. https://doi.org/10.33084/jsm.v6i1.1630
Harjuna Asrul, Mallapiang Fatmawaty, & Idris Prihatin, F. (2019). Efektivitas Pemberian Madu Terhadap Peningkatan Hemoglobin Pada Pekerja Wanita di PT. Maruki International Indonesia. Jurnal Ilmiah Kesehatan Diagnosis, 13(9), 633–637. http://jurnal.stikesnh.ac.id/index.php/jikd/article/view/108
Hayati, . E., Sari, & Husna. (2024). Pengaruh Pemberian Sayur Kelakai (Stenochlaena Palustris) Terhadap Peningkatan Kadar Hemoglobin Pada Ibu Hamil Di Klinik Citra Marindal Tahun 2024. Jurnal Pengabdian Masyarakat Putri Hijau, 4(3), 45–48. https://doi.org/10.36656/jpmph.v4i3.1851
Kesuma Candra, Murjani, & Basyid. (2017). Pengaruh Ekstrak Kelakai (Stenochlaena palustris) Terhadap Kadar Hemoglobin Pada Tikus Putih (Rattus norvegicus). Borneo Journal of Pharmascientech, 1(1), 10–17.
Kustiawan, P. M., Nur, D., Hanifa, C., Syahrian, A., Nugraha, D., Suwandi, A., Monica, A., Agustinur, A., & Timur, K. (2023). Edukasi dan Pelatihan Pembuatan Turunan Hasil Olahan dari Produk Lebah Stingless bee pada Kelompok Peternak Lebah di Samarinda. Jurnal Ilmiah Pengabdian Kepada Masyarakat Volume, 8(1), 21–26.
Nafisah, Habibah, Sarmila, Setiawati, Saputri, & Komar. (2022). Potensi Senyawa Kimia dari Herba Kelakai ( Stenochlaena palustris ) sebagai Antikanker : Kajian Molecular Docking. Prosiding Seminar Nasional Kimia, 15(December), 39–45. https://doi.org/10.15575/gdcs.v15i
Pamela, Ayu, D. D., Nurmala, Ira, Ayu, & Sekar, R. (2022). Faktor Risiko Dan Pencegahan Anemia Pada Wanita Usia Subur Di Berbagai Negara. Ikesma, 18(3), 161. https://doi.org/10.19184/ikesma.v18i3.26510
Putri, Purnama, D., Pujiastuti, Endang, S., Ramlan, & Djamaluddin. (2023). Teh Daun Kalakai (Stenochlaena Palustris) untuk Meningkatkan Kadar Hemoglobin pada Remaja Putri Anemia. Jurnal Kebidanan UM Mataram, 7(2), 26–28.
Rahayu, A., Yulidasari, F., Putri, A. O., & Anggraini, L. (2019). Buku Referensi: Metode Orkes-Ku (raport kesehatanku) dalam Mengidentifikasi Potensi Kejadian Anemia Gizi pada Remaja Putri. In CV Mine.
Rianti, P. T., Sukarni, Utami, L. T., & Febriyanti, H. (2022). Pengaruh Madu Terhadap Kadar Hemoglobin Pada Ibu Hamil. Jurnal Maternitas Aisyah (Jaman Aisyah), 3(2), 159–165. https://doi.org/10.30604/jaman.v3i2.486
Rista Yuziani. (2015). Efektivitas Madu Terhadap Peningkatan HB Pada Tikus Putih. JESBIO, III(5), 7–13.
Risyad, M Naufal, Fauziah, Nurul, & Hidayah. (2022). Pengaruh Konsumsi Kelakai (Stenochlaena palustris) Terhadap Anemia Pada Kehamilan Dan Risiko Bayi Berat Lahir Rendah. Lambung Mangkurat Medical Seminar, 3(1), 118–124.
Rizky, M. M., Muris, S. I., & Kustiawan Paula Mariana. (2025). Aktivitas Antiinflammasi Kombinasi Ekstrak Propolis dan Madu Lebah Heterotrigona itama pada Mencit (Mus musculus) yang diinduksi Karagenan. Healthy-Mu Journal, 9(1), 34–41. https://doi.org/10.35747/hmj.v9i1.1314
Rusmiati, D. (2019). Zat Besi Dengan Dan Tanpa Vitamin C Terhadap Kenaikan Kadar Hemoglobin Ibu Hamil. Jurnal Ilmiah Bidan, 4(2), 30–35.
Suryawan, N. W., Bachrun, E., Prayitno, S., Bhakti, S., Mulia, H., Relationship, T., Parenting, B., & Behavior, S. (2023). Gambaran Pola Makan dengan Kejadian Anemia. JPKM Jurnal Profesi Kesehatan Masyarakat, 4(1), 1–7.
Syamsul, Siswanto, E., Hakim, Yunita, Y., Nurhasnawati, & Henny. (2019). Penetapan Kadar Flavonoid Ekstrak Daun Kelakai (Stenochlaena palustris (Burm. F.) Bedd.) Dengan Metode Spektrofotometri UV-Vis. Jurnal Riset Kefarmasian Indonesia, 1(1). https://doi.org/10.33759/jrki.v1i1.46
Syamsul, Tri, Damayanty, Lala, & Syaharuddin. (2022). Kandungan Fitokimia , Polifenol Dan Flavonoid Madu Trigona. Journal Of Training And Community Service Adpertisi, 2(2), 62–70.
Wijianto, B., Pratiwi, L., & Hermawati, E. (2025). Evaluation of Physicochemical and Antioxidant Properties of Stingless bee (Heterotrigona itama) Honey and Forest Honey Authentic to West Borneo. Letters in Applied NanoBioScience, 14(1), 1–8. https://doi.org/10.33263/LIANBS141.042
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Salsa Hermadhiyaa Jauzaa, Paula Mariana Kustiawan
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
