The Effect of Extraction Solvents towards Total Flavonoid Content and Total Phenolic Content of Butterfly Pea (Clitoria ternatea) Extract

Authors

  • Endah Kurniawati Universitas Jenderal Achmad Yani Yogyakarta
  • Nanda Tsalasani Zulfaidah Pharmacy Department, Universitas Jenderal Achmad Yani, Yogyakarta
  • Siti Nur Amaliya Pharmacy Department, Universitas Jenderal Achmad Yani, Yogyakarta
  • Siska Putri Kurniawati Pharmacy Department, Universitas Jenderal Achmad Yani, Yogyakarta

Keywords:

Clitoria ternatea, Phenolic, Flavonoid, TPC, TPF

Abstract

Butterfly pea flower (Clitoria ternatea) has many benefits including antioxidant. The antioxidant activity was contributed by flavonoid and phenolic components. The choice of solvent is important to obtain the metabolite components that contribute to antioxidant activity. Therefore this study was conducted to find the ability of different solvent to obtain total flavonoid content (TFC) and total phenolic content (TPC) in C. ternatea. The fine powder of dried flower was extracted by different solvents (ethanol 70%, ethanol 96%, and methanol). Quercetin was used as standard in determination of TFC and gallic acid was used as standard in determination of TPC. The TFC value of ethanol 70%, ethanol 96% and methanol extract were 46,23±0,39; 28,19±0,03; and 16,87±0,11 mg QE/g extract respectively. The TPC value of ethanol 70%, ethanol 96% and methanol extract were 93,91±0,16; 78,63±0,16; 84,46±0,16 mg GAE/g extract respectively. Ethanol 70% extract had the highest yield value and the highest amount of TPC and TFC compare to other solvents. Thus, the amount of flavonoid and phenolic in ethanol 70% extract showed the promising potential benefit of C. ternatea as antioxidant.

References

Abeysinghe, D. T., Kumara, K. A. H., Kaushalya, K. A. D., Chandrika, U. G., & Alwis, D. D. D. H. (2021). Phytochemical screening, total polyphenol, flavonoid content, in vitro antioxidant and antibacterial activities of Sri Lankan varieties of Murraya koenigii and Micromelum minutum leaves. Heliyon, 7(7). https://doi.org/10.1016/j.heliyon.2021.e07449.

Andriani, D., & Murtisiwi, L. (2018). Penetapan Kadar Fenolik Total Ekstrak Etanol Bunga Telang (Clitoria Ternatea L.) Dengan Spektrofotometri UV Vis. Cendekia Journal of Pharmacy, 2(1). https://doi.org/10.31596/cjp.v2i1.15.

Aryal, S., Baniya, M. K., Danekhu, K., Kunwar, P., Gurung, R., & Koirala, N. (2019). Total Phenolic content, Flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants, 8(4). https://doi.org/10.3390/plants8040096.

Bendary, E., Francis, R. R., Ali, H. M. G., Sarwat, M. I., & El Hady, S. (2013). Antioxidant and structure–activity relationships (SARs) of some phenolic and anilines compounds. Annals of Agricultural Sciences, 58(2). https://doi.org/10.1016/j.aoas.2013.07.002.

Chen, J., Ning, S., Lu, X., Xiang, W., Zhou, X., Bu, Y., Li, L., & Huang, R. (2023). Variation in flavonoid and antioxidant activities of Pyrrosia petiolosa (Christ) Ching from different geographic origins. Frontiers in Plant Science, 14. https://doi.org/10.3389/fpls.2023.1173489.

Chen, S., Wang, X., Cheng, Y., Gao, H., & Chen, X. (2023). A Review of Classification, Biosynthesis, Biological Activities and Potential Applications of Flavonoids. In Molecules (Vol. 28, Issue 13). https://doi.org/10.3390/molecules28134982.

Do, Q. D., Angkawijaya, A. E., Tran-Nguyen, P. L., Huynh, L. H., Soetaredjo, F. E., Ismadji, S., & Ju, Y. H. (2014). Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3). https://doi.org/10.1016/j.jfda.2013.11.001.

Fattahi, S., Zabihi, E., Abedian, Z., Pourbagher, R., Motevalizadeh Ardekani, A., Mostafazadeh, A., & Akhavan-Niaki, H. (2014). Total Phenolic and Flavonoid Contents of Aqueous Extract of Stinging Nettle and In Vitro Antiproliferative Effect on Hela and BT-474 Cell Lines. International Journal of Molecular and Cellular Medicine, 3(2), 102–107.

Hajji Nabih, M., Boulika, H., El Hajam, M., Alghonaim, M. I., Kandri, N. I., Alsalamah, S. A., & Boufahja, F. (2023). Successive Solvent Extraction, Characterization and Antioxidant Activities of Cardoon Waste (Leaves and Stems) Extracts: Comparative Study. Molecules, 28(3). https://doi.org/10.3390/molecules28031129.

Hikmawanti, N. P. E., Fatmawati, S., & Asri, A. W. (2021). The effect of ethanol concentrations as the extraction solvent on antioxidant activity of Katuk (Sauropus androgynus (L.) Merr.) leaves extracts. IOP Conference Series: Earth and Environmental Science, 755(1). https://doi.org/10.1088/1755-1315/755/1/012060.

Jaafar, N. F., Ramli, M. E., & Salleh, R. M. (2020). Optimum extraction condition of Clitorea ternatea flower on antioxidant activities, total phenolic, total flavonoid and total anthocyanin contents. Tropical Life Sciences Research, 31(2), 1–17. https://doi.org/10.21315/tlsr2020.31.2.1.

Jeyaraj, E. J., Lim, Y. Y., & Choo, W. S. (2021). Extraction methods of butterfly pea (Clitoria ternatea) flower and biological activities of its phytochemicals. Journal of Food Science and Technology, 58(6), 2054–2067. https://doi.org/10.1007/s13197-020-04745-3.

Mehmood, A., Javid, S., Khan, M. F., Ahmad, K. S., & Mustafa, A. (2022). In vitro total phenolics, total flavonoids, antioxidant and antibacterial activities of selected medicinal plants using different solvent systems. BMC Chemistry, 16(1). https://doi.org/10.1186/s13065-022-00858-2.

Mehrabani, M., Lotfian sargazi, M., Amirkhosravi, A., Farhadi, S., Vasei, S., Raeiszadeh, M., & Mehrabani, M. (2023). The influence of harvest time on total phenolic and flavonoid contents, antioxidant, antibacterial and cytotoxicity of Rheum khorasanicum root extract. Annales Pharmaceutiques Francaises, 81(3). https://doi.org/10.1016/j.pharma.2022.11.010.

Oguis, G. K., Gilding, E. K., Jackson, M. A., & Craik, D. J. (2019). Butterfly pea (Clitoria ternatea), a cyclotide-bearing plant with applications in agriculture and medicine. Frontiers in Plant Science, 10. https://doi.org/10.3389/fpls.2019.00645.

Panche, A. N., Diwan, A. D., & Chandra, S. R. (2016). Flavonoids: An overview. Journal of Nutritional Science, 5. https://doi.org/10.1017/jns.2016.41.

Pandey, K. B., & Rizvi, S. I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, 2(5), 270–278. https://doi.org/10.4161/oxim.2.5.9498.

Pérez, M., Dominguez-López, I., & Lamuela-Raventós, R. M. (2023). The Chemistry Behind the Folin-Ciocalteu Method for the Estimation of (Poly)phenol Content in Food: Total Phenolic Intake in a Mediterranean Dietary Pattern. Journal of Agricultural and Food Chemistry, 71(46), 17543–17553. https://doi.org/10.1021/acs.jafc.3c04022.

Puspa Yani, N. K. L., Nastiti, K., & Noval, N. (2023). Pengaruh Perbedaan Jenis Pelarut Terhadap Kadar Flavonoid Total Ekstrak Daun Sirsak (Annona muricata L.). Jurnal Surya Medika, 9(1). https://doi.org/10.33084/jsm.v9i1.5131.

Shraim, A. M., Ahmed, T. A., Rahman, M. M., & Hijji, Y. M. (2021). Determination of total flavonoid content by aluminum chloride assay: A critical evaluation. LWT - Food Science and Technology, 150. https://doi.org/10.1016/j.lwt.2021.111932.

Sultana, B., Anwar, F., & Ashraf, M. (2009). Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules, 14(6). https://doi.org/10.3390/molecules14062167.

Tungmunnithum, D., Thongboonyou, A., Pholboon, A., & Yangsabai, A. (2018). Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines, 5(3). https://doi.org/10.3390/medicines5030093.

Vidana Gamage, G. C., Lim, Y. Y., & Choo, W. S. (2021). Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications. In Frontiers in Plant Science (Vol. 12). Frontiers Media S.A. https://doi.org/10.3389/fpls.2021.792303.

Vifta, R. L., Trinadi, K. S., & Suratno, S. (2022). Potential of Flavonoid Content from Clitoria ternatea Flowers Extract as Natural Antioxidant Candidate and Its Correlation. The 1st International Conference on Health, Faculty of Health.

Waruwu, I. S., Rawar, E. A., & Kristiyani, A. (2023). Penetapan Kadar Flavonoid Total Dan Fenolik Total Serta Uji Penghambatan Denaturasi Protein Dalam Seduhan Teh Bunga Telang (Clitoria ternatea L.). Majalah Farmasi Farmakologi, 27(2).

Zhang, Y., Cai, P., Cheng, G., & Zhang, Y. (2022). A Brief Review of Phenolic Compounds Identified from Plants: Their Extraction, Analysis, and Biological Activity. In Natural Product Communications (Vol. 17, Issue 1). https://doi.org/10.1177/1934578X211069721.

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2024-12-31

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