The Effect of Ethanol Concentration Variations on The Total Phenolic And Flavonoid Levels of Bauhinia purpurea L. Leaf Extract
Keywords:
Bauhinia purpurea, 70% Ethanol, 90% Ethanol, Phenolic, FlavonoidAbstract
Bauhinia purpurea L. is an ornamental plant that is not widely known or explored by the public, despite its significant antioxidant properties. Phenolic compounds and flavonoids, known for their antioxidant activity, can be extracted from the leaves through maceration with 70% ethanol and 96% ethanol. The difference in solvent polarity between these two ethanol concentrations affects the solubility of bioactive compounds. This study aimed to evaluate the effect of 70% and 96% ethanol concentrations Bauhinia purpurea L on the total phenolic and flavonoid levels in. An experimental method was employed, using Bauhinia purpurea L powder macerated with 70% and 96% ethanol, followed by evaporation at 40°C-50°C to obtain thick extracts. The extracts were subjected to organoleptic tests, phytochemical screening, Thin Layer Chromatography (TLC), and UV-Vis spectrophotometry. Total phenolic content was determined using gallic acid standards (mg GAE/g extract), while total flavonoid content was measured using quercetin standards (mg QE/g extract). The organoleptic test revealed that both the 70% and 96% ethanol extracts of Bauhinia purpurea L had a blackish-green color, thick consistency, distinctive odor, and bland taste. Phytochemical screening indicated the presence of phenolics, flavonoids, and saponins. The total phenolic content for the 70% ethanol extract was 14.644 ± 0.222 mg GAE/g, and the total flavonoid content was 25.519 ± 0.921 mg QE/g. The total phenolic content for the 96% ethanol extract was 7.176 ± 0.347 mg GAE/g, and the total flavonoid content was 11.208 ± 0.412 mg QE/g. These results indicate a significant difference between the use of 70% and 96% ethanol in extracting total phenolic and flavonoid content from Bauhinia purpurea L, with the 70% ethanol extract showing higher levels of both compounds.
References
Ahmad, A. R., Sakinah, Wisdawati, & Asrifa, W. O. (2014). Study of antioxidant activity and determination of phenol and flavonoid content of pepino’s leaf extract (Solanum muricatum aiton). International Journal of PharmTech Research, 6(2), 600–606.
Aryantini, D. (2021). Aktivitas Antioksidan dan Kandungan Tanin Total Ekstrak Etanol Daun Kupu-Kupu (Bauhinia purpurea L.). Diakses pada 10 Januari 2024 pukul 15.00 WIB. Jurnal Farmagazine, 8(1), 54. https://doi.org/10.47653/farm.v8i1.537
Chang, C. C., Yang, M. H., Wen, H. M., & Chern, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colometric methods. Journal of Food and Drug Analysis, 10(3), 178–182. https://doi.org/10.38212/2224-6614.2748
Chun, O. K., Kim, D. O., & Lee, C. Y. (2003). Superoxide Radical Scavenging Activity of the Major
Polyphenols in Fresh Plums. Journal of Agricultural and Food Chemistry, 51(27), 8067–8072.
https://doi.org/10.1021/jf034740d
Djuleng, A. (2021). Identifikasi Senyawa Total Fenolik Dan Total Flavonoid Ekstrak Larut Etanol Daun Kupu-Kupu (Bauhinia purpurea) Dengan Spektrofotometri UV-Vis. Universitas Jenderal Achmad Yani Yogyakarta.
Harbone, J. B. (1997). Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan (S. I. Penerjemah:Padmawinata K (ed.); 3rd ed.). ITB.
Kementrian Kesehatan RI. (2017). Farmakope Herbal Indonesia (II). Kementrian Kesehatan RI.
Khairunnisa, S., Hakim, A. R., & Audina, M. (2022). Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol Dari Ekstrak Daun Pegagan (Centella asiatica [L] Urban). Journal Pharmaceutical Care and Sciences, 3(1), 121–131. https://doi.org/10.33859/jpcs.v3i1.236
Krishnaveni, M. (2014). Antioxidant potential of bauhinia purpurea (L) leaf. International Journal of Pharmacy and Pharmaceutical Sciences, 6(7), 558–560.
Masúd, F., & Puspitasari, P. (2017). Studi Pendahuluan Ekstraksi Bertingkat Minyak Biji Mangga Arumanis (Mangifera Indica) Menggunalan Pelarut N-Heksan dan Etanol. INTEK: Jurnal Penelitian, 4(1), 42. https://doi.org/10.31963/intek.v4i1.92
Minarno, B. E. (2015). Skrining Fitokimia dan Kandungan Total Flavanoid Pada Buah Carica pubescens Lenne & K. Koch Di Kawasan Bromo, Cangar, dan Dataran Tinggi Dieng. El-Hayah, 5(2), 73–82. https://doi.org/10.18860/elha.v5i2.3022
Mukhriani, M., Rusdi, M., Arsul, M. I., Sugiarna, R., & Farhan, N. (2019). Kadar Fenolik dan Flavonoid Total Ekstrak Etanol Daun Anggur (Vitis vinifera L). Ad-Dawaa’ Journal of Pharmaceutical Sciences, 2(2), 95–102. https://doi.org/10.24252/djps.v2i2.11503.s
Oktavia, F. D., & Sutoyo, S. (2021). Skrining Fitokimia, Kandungan Flavonoid Total, Dan Aktivitas
Antioksidan Ekstrak Etanol Tumbuhan Selaginella doederleinii. Jurnal Kimia Riset, 6(2), 141. https://doi.org/10.20473/jkr.v6i2.30904.
Oktaviantari, D. E., Feladita, N., & Agustin, R. (2019). Identifikasi Hidrokuinon Dalam Sabun Pemutih Pembersihwajah Pada Tiga Klinik Kecantikan Di Bandar Lampung Dengan Metode Kromatografi Lapis Tipis dan Spektrofotometri UV-Vis. Jurnal Analis Farmasi , 4(2), 91–97.
Pratiwi, D. N., Utami, N., & Pratimasari, D. (2022). Karakterisasi dan Penetapan Kadar Flavonoid Total Ekstrak dan Fraksi Bunga Pepaya Jantan (Carica papaya L.) dengan Spektrofotometri UV-Vis. Jurnal Ilmiah Farmasi, 18(2), 219–233. https://doi.org/10.20885/jif.vol18.iss2.art20
Pujiastuti, E., & El’Zeba, D. (2021). PERBANDINGAN KADAR FLAVONOID TOTAL EKSTRAK 70%
ETHANOL DAN 96% KULIT BUAH NAGA MERAH Hylocereus polyrhizus) DENGAN SPEKTROFOTOMETRI. Cendekia Journal of Pharmacy, 5(1), 28–43. https://doi.org/10.31596/cjp.v5i1.131.
Purwasari, F. (2021). Uji Peredaman Radikal Bebas DPPH (2.2 diphenyl-1-pikrilhidrazil) Ekstrak Etanol Daun Kupu-kupu (Bauhinia purpurea L.). Universitas Jenderal Achmad Yani Yogyakarta.
Suhendra, C. P., Widarta, I. W. R., & Wiadnyani, A. A. I. S. (2019). Pengaruh Konsentrasi Etanol Terhadap Aktivitas Antioksidan Ekstrak Rimpang Ilalang ( (Imperata cylindrica (L) Beauv.) pada Ekstraksi Menggunakan Gelombang Ultrasonik. Jurnal Ilmu Dan Teknologi Pangan, 8(1), 27–35.
Yusharyahya, S. N. (2021). Mekanisme Penuaan Kulit sebagai Dasar Pencegahan dan Pengobatan Kulit Menua. EJournal Kedokteran Indonesia, 9(2), 150. https://doi.org/10.23886/ejki.9.49.150
Wahyudi, T. A., & Minarsih, T. (2023). Pengaruh Ekstraksi dan Konsentrasi Etanol terhadap Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Jahe Emprit (Zingiber officinale var. Amarum). Indonesian Journal of Pharmacy and Natural Product, 06(01), 30–38. https://doi.org/10.35473/ijpnp.v6i01.2208.
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