Vinca alkaloids effects on the morphological characters of Canavalia ensiformis
DOI:
https://doi.org/10.12928/jbns.v2i1.6253Keywords:
Jack bean, periwinkle, polyploidy, vinca alkaloidsAbstract
Productivity and quality of crops can be increased through polyploidy plants induced by antimitotic compounds such as vinka alkaloids.The use of vinka alkaloids has never been applied to Sword bean (Canavalia ensiformis), a tropical legume belonging to the Fabaceae family. This study aimed to determine the effects of vinka alkaloids treatment on the morphological characters of Sword bean. The treatment consist of four different vinka alkaloids concentrations (0.5%, 1.00%, 1.50%, 2.0%, and untreated (0.0%) act as control. The plants were arranged in a completely randomized design. Treated plants were characterized based on morphological traits such as plant height, leaf area and dry seed weight. The results obtained revealed significant difference (p≤0.05) in the morphological traits of the treated plants when compared with the control. Overall, 2.0% vinka alkaloids concentration resulted in higher average plant height by 193.8 ± 1.86, leaf area increments by 88.6 ± 5.1 (left leaf), 115.1 ± 4.7 (middle leaf), 86.2 ± 3.8 (right leaf) and the dry seed weight by 1.7 ± 3.6. The improvement of the traits is concentration dependent and increases with increment in vinka alkaloids concentration. Thus, we suggested that 2.0% concentration should be employed in improving Sword bean growth and yield.
References
Breseghello, F. and Coelho, A.S.G. (2013). Traditional and modern plant breeding methods with examples in rice (Oryza sativa L.). J Agric Food Chem 61: 8277–8286. https://doi.org/10.1021/jf305531j
BPS. 2021. Impor kedelai menurut negara asal utama 2010-2019. Diakses dari https://www.bps.go.id/statictable/2019/02/14/2015/impor-kedelai-menurut-negara-asal-utama-2010-2019.html. Pada tanggal 28 Mei 2022.
Chung, I.M., Kim, E.H., Li, M., Peebles, C.A.M., Jung, W.S, Song, H.K., Ahn, J.K., dan San, K.Y. (2011). Screening 64 Cultivars Catharanthus roseus for the Productioins of Vindoline, Catharanthine, and Serpentine. Biotechnol Prog. 27(4): 937-943.
Daryono, B.S. 2015. Serbuk Tanaman Tapak Dara (Catharanthus roseus (L.) G. DON.) dan Penggunaannya Sebagai Agensia Poliploidisasi Tanaman. Yogyakarta: UGM.
Daryono, B. S., Nofriarno, N., Saputri, A. P., & Indraningsih, E. (2018). Analisis fenotipe dan ploidi tanaman melon (Cucumis melo L.) hasil perlakuan ekstrak etanolik daun tapak dara (Catharanthus roseus [L] G. Don.).Jurnal Biota, 4(2), 62-67
Hadfield, J., Ducki, S., Hirst, N., & McGown, A. (2014). Tubulin and Microtubules as Target for Anticancer Drugs. Progress in Cell Cycle Research, 5: 309-325.
Iskandar, N.N. dan Iriawati. (2016). Vinblastine and Vincristine Production on Madagascar Periwinkle (Catharanthus roseus L.) Callus Treated with Polethylene Glycol. Makara Journal of Science, 20 (1). p: 7-16.
Kamwean P, Chaisan T, Thobunluepop P et al. (2017). Changing of morphological characteristic and biomass properties in Pennisetum purpureum by colchicine treatment. J Agron, 16:23–31. https://doi.org/10.3 923/ja.2017.23.31
Munzbergova, Z. (2017). Colchicine application significantly affects plant performance in the second generation of synthetic polyploids and its effects vary between populations. Ann Bot, 120:329–339. https://doi.org/10.1093/aob/mcx070
Purbosari, P. P., and Etika, D. P. (2018). Pengaruh Ekstrak Etanol Daun Tapak Dara (Catharanthus roseus L.) dan Kolkisin Terhadap Perkecambahan Biji Cabai Rawit Hibrida (Capsicum annum). BIOEDUKASI. 9(2).
Rahayu, Y.S.S., I.K. Prasetyo, A.U. Riada. (2014). Pengaruh penggunaan kolkisin terhadap pertumbuhan vegetatif tanaman sedap malam (Polianthes tuberose L.) di dataran medium. Agromix 5 (1) 44-56.
Solomon S. G., Okomoda V. T. and Oguche O. (2017). Nutritional value of raw Canavalia ensiformis and its utilization as partial replacement for soybean meal in the diet of Clarias gariepinus (Burchell, 1822) fingerlings. Food Sci. Nut. 6(1) 207–13.
Suciati, A. 2012. Pengaruh Lama Perendaman dan Fermentasi Terhadap Kandungan HCN Pada Tempe Kacang Koro (Canavalia ensiformis L.). Makassar : Universitas Hasanuddin.
Tammu, R.M., Nuringtyas, T.R. & Daryono, B.S. (2021) Colchicine effects on the ploidy level and morphological characters of Katokkon pepper (Capsicum annuum L.) from North Toraja, Indonesia. J Genet Eng Biotechnol 19, 31. https://doi.org/10.1186/s43141-021-00131-4
Viza, R.Y. (2019). Karakteristik Morfologi Tanaman Mentha spicata Hasil Induksi Ekstrak Etanolik Daun Tapak Dara (Catharanthus roseus). Jurnal Pendidikan Biologi dan Biosains. 2(1): 15-20.
Wardana, A. Slamet, S.H. Andarias, A.H. Bahrun, K. Manja dan Darwis. 2019. Induction of Lili Hujan Polyploid (Zephyranthes rosea Lindl.) with Ethanolic Extract of Tapak Dara Leaf (Catharanthus roseus (L.) G. Don.) to Increase it’s Economic Value. IOP Conference Series: Earth and Environmenal Science.
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