Journal of Biotechnology and Natural Science

Identification of Phytochemical Compounds and Determination of Flavonoid and Tannin Levels in Wedelia (Sphagneticola trilobata (L.) Pruski)

Haris Setiawan — 2025-06-30

Wedelia plant, which has the scientific name Sphagneticola trilobata (L.) Pruski, is one of the plants classified as weeds and is found in various agricultural areas. The largest phytochemical compound content in wedelia leaves consists of flavonoids, tannins and alkaloids. The purpose of this study was to identify phytochemical compounds contained in wedelia leaves and to determine the levels of existing compounds. This research method consists of wet sorting, making extracts by maceration method using 96% ethanol. The ethanol extract was then analyzed using Fourier Transform InfraRed (FT-IR), High Performance Liquid Chromatography (HPLC), and UV-Vis Spectrophotometer. Data analysis to determine the line equation and correlation value using statistical analysis application SPSS Version 30.0 and analysis of phytochemical compound components using descriptive analysis. Based on the results of the analysis of phytochemical compounds using the FTIR method, several functional groups were obtained that were very identical to antioxidant compounds in the form of flavonoids, tannins, and alkaloids. After that, further tests on flavonoid levels using the HPLC method obtained levels of 215.435 mg/g (%b/b), and determination of tannin levels using a UV-Vis Spectrophotometer obtained levels of 128.35 mg/g (%b/b).

Preliminary Survey of Aedes sp. Larvae in Mosques of Banda Aceh During Ramadan 2025

Muhammad Farhan Putra Emil — 2025-06-30

The Aedes sp. mosquito is the main vector of dengue fever that breeds in environments with suitable breeding sites. Mosques, as places of worship with bathroom facilities and water storage containers, have the potential to become breeding grounds for Aedes sp. mosquitoes. This study aims to identify the presence of Aedes sp. larvae in mosque bathrooms in Banda Aceh during Ramadan 2025. A total of 45 mosques across nine districts were surveyed using a random sampling method (data representation with equal chances of selection). Observations focused on water storage containers, ventilation, lighting, and the availability of container covers. Larvae and water samples were collected and analyzed in the laboratory. The findings confirm the presence of Aedes aegypti and Aedes albopictus larvae, with a higher prevalence of Aedes aegypti due to its preference for indoor breeding sites. Poor ventilation, inadequate lighting, and uncovered water storage containers were key factors contributing to mosquito breeding. These results highlight the potential risk of dengue transmission in mosques, emphasizing the need for improved water management and preventive measures to ensure worshippers' safety. Strengthening mosquito control efforts in places of worship is essential to minimize dengue fever outbreaks, particularly during periods of increased human activity such as ramadan.

Characteristics and Diversity of Riparian Plant Species in Relation to Plastic Litter Entrapment in the Bedog River, Indonesia

Inggita Utami — 2025-06-30

The increasing production of waste and inadequate waste management on land have led to significant plastic leakage into river systems. Riparian vegetation is now increasingly covered by plastic debris, posing a threat to essential biological processes such as pollination and plant metabolism. The aim of this study is to identify the characteristics and types of riparian plant species that trap plastic litter along the riparian zones. The research was conducted in Indonesia, specifically in Yogyakarta Province, which is currently facing a waste management crisis. Bedog River was selected as the sampling site due to its course through densely populated areas, including Sleman and Bantul Regencies. A total of 20 sampling plots, spaced one to two kilometers apart, were distributed along the river, with each plot containing a 25-square-meter sampling plot designated for plant identification. In total, 78 plant species, predominantly from the Poaceae, Moraceae, and Fabaceae families, were found to trap plastic debris. Branches and twigs were the most frequently affected plant structures. Bamboo and shrubs exhibited distinctive morphological characteristics that facilitated plastic entrapment, particularly of small plastic fragments. A substantial portion of riparian vegetation along the Bedog River is covered with plastic waste, necessitating targeted conservation efforts for affected species.

Antibiotic Resistance in Escherichia coli Isolated from Chicken Meat in Traditional Markets of Yogyakarta

Irsyad Al Farisi — 2025-06-30

The widespread use of antibiotics in the livestock industry serves both as a preventive measure against diseases and as a feed additive. However, excessive antibiotic use has led to antibiotic resistance, where bacterial exposure to antibiotics renders them resistant to one or more antibiotic agents. This study investigates the presence of antibiotic resistance in Escherichia coli isolated from chicken meat sold in traditional markets in Yogyakarta. An experimental method was employed, using ECB and EMBA media for bacterial isolation. Gram staining was performed to characterize the morphology of E. coli, followed by molecular testing using the uspA gene. Antibiotic susceptibility testing was conducted using the Kirby-Bauer disk diffusion method. A total of 10 chicken meat samples were collected from five traditional markets in Yogyakarta. The antibiotics tested included ampicillin, ciprofloxacin, and chloramphenicol. The results indicated resistance in all 10 isolates. Sample codes G1 and G2 were resistant to all three antibiotics. Samples P1, P2, and L1 exhibited resistance to ampicillin and chloramphenicol, with intermediate resistance to ciprofloxacin. Sample L2 showed resistance to ampicillin, intermediate resistance to ciprofloxacin, and sensitivity to chloramphenicol. Sample B1 exhibited resistance to all antibiotics tested. Sample B2 was resistant to ampicillin and ciprofloxacin, with intermediate resistance to chloramphenicol. Samples K1 and K2 were resistant to ampicillin and chloramphenicol but sensitive to ciprofloxacin. These findings highlight the growing concern of antibiotic resistance in bacterial strains isolated from chicken meat, emphasizing the need for stricter regulations on antibiotic use in the poultry industry.

The Effect of Extraction Method on Total Flavonoid Content of Hedyotis corymbosa L.

Rengganis Ulvia — 2025-06-30

Hedyotis corymbosa L. (H. corymbosa) is an Indonesian herbal plant with many health benefits. This activity comes from secondary metabolite compounds, one of which is flavonoids. These compounds can be obtained through an extraction process, where the extraction method is one of the factors that can affect the levels of compounds. This study aims to determine the effect of conventional extraction methods: maceration and soxhletation with non-conventional methods, Ultrasound Assisted Extraction (UAE) on the total flavonoid content of H. corymbose. The sample was extracted using 70% ethanol solvent (1:10 w/v) with the maceration, soxhletation, and UAE methods. The total flavonoid content of the H. corymbosa extract was measured using UV-Vis spectrophotometry. The data obtained were then analyzed statistically using SPSS One-Way ANOVA, followed by Post Hoc Tukey with a 95% confidence level. The soxhletation method has the highest flavonoid content at 72.255±1.334 mg QE/g, followed by UAE at 69.118±1.782 mg QE/g, and maceration at 43.725±0.679 mg QE/g. Statistical analysis confirmed that the extraction method significantly influences total flavonoid content. While both soxhletation and UAE methods produced similarly high flavonoid contents, UAE offers a substantial advantage in efficiency due to its shorter extraction time, making it a promising alternative to conventional soxhletation.

Ethnopharmacological Study of Medicinal Plants in Kedungpoh Village, Nglipar Sub-district, Gunungkidul District, Special Region of Yogyakarta

Ludmilla Fitri Untari — 2025-06-30

The scientific study of ethnopharmacology investigates the medicinal uses of plants by different ethnic groups and plays a crucial role in the early-stage drug discovery process by identifying potential drug candidates from traditional remedies. The transmission of knowledge concerning the diversity of plant species and their utilization is typically an intergenerational process, facilitating the preservation of cultural heritage and the transfer of experiential knowledge. The present study was conducted in the village of Kedungpoh, Nglipar, Gunungkidul. The objective of this study is to investigate the utilisation, management and comprehension of medicinal plants within the Kedungpoh village in Gunungkidul.The research methodology comprised of observational studies and interviews with local residents. The results revealed the utilization of 24 plant species, which classified into 17 distinct families by the local community. The utilization of these plants encompassed various plant organs, including leaves, stems, roots, fruits, and rhizomes, which were employed in different processing methods, such as crushing, boiling, or rubed. The most prevalent and utilized plant families were Asteraceae, which are notable for their abundance of bioactive compounds, including flavonoids, alkaloids, and essential oils, which have been demonstrated to be efficacious in addressing a wide range of health concerns. This study provides substantial support for scientific laboratory research into the safety, quality and efficacy of traditional medicine practices, with a view to further utilization of traditional medicine, whilst also protecting the intellectual property rights of traditional knowledge holders.